YTHDF1 and cancer: While according to ELISA assay, the levels of secreted interferon α/β/γ proteins in HGC27 were slightly upregulated by METTL14/YTHDF1 knockdown, or downregulated by FTO knockdown (Figure S4E), which was in accordance with the findings of Winkler et al.29 Since interferons exerted dual functions in mediating cancer immunity, m6A may modulate immune responses of GC through repressing interferon production (Figure 5C).