Consistent with our observation in IGH-DUX4 B-ALL patient samples, DUX4 expression in Nalm6 is much higher compared with its normal expression in human cleavage stage embryos (FPKM 41.3 vs. 6.65, Fig. 1a) and much lower than Igμ (FPKM 41.3 vs. 638.2, Fig. 1b), making the cell line a good model for evaluating allele specificity of the IGH-DUX4 translocation. The gene discussed is DUX4; the disease is acute lymphoblastic leukemia.