Eight of the recombinant C. crescentus bacteria (those expressing Fuzeon [Cc-Fuzeon], T1249 [Cc-T1249], C52 [Cc-C52], CD4 [Cc-CD4], CD4M33F23 [Cc-CD4M33F23], MIP-1α [Cc-MIP1α], indolicidin [Cc-indolicidin], and BmKn2 [Cc-BmKn2]) provided some level of protection from vaginal infection with HIV-1, based on both p24 ELISA and RT-qPCR results, although this protection was not statistically significant (Fig. 6c to j). This evidence concerns the gene CD4 and infective vaginitis.