Infection with EPEC-espF+EspF and EPEC-map+Map evoked a sharp decrease in perinuclear Tfn-positive endosomal area (Fig 8A and 8B), a concomitant increase in Tfn/TfnR and Tfn/EEA1 intensity in the peripheral endosomes (Fig 8C and 8D), and an increase in Tfn endocytic turnover (Fig 8E and 8F). Here, BDP1 is linked to infection.