We therefore investigated the T3SS dependence of the distribution of clathrin endocytic (CHC, AP2-α, Rab5a and EEA1) and recycling [Rab11a, Rab11b, Rab25, Myosin 5b (Myo5b)] components upon EPEC infection of polarized MDCK cells. This evidence concerns the gene EEA1 and infection.