Our results show that EPEC type III secreted components stimulate the recruitment of clathrin-dependent early (Rab5a, EEA1) and recycling (e.g. Rab11a, Rab11b, Rab4a, Rab25, Myo5b, FIP2) endocytic machineries to plasma membrane infection sites of polarized and non-polarized epithelial cells (S1, S4A and S4B Figs). Here, RAB25 is linked to infection.