Heim et al. placed ApoE-/- hyperlipidemic mice on a high fat diet (HFD containing 1% cholesterol, 7% fat, and 1% cholate), intraperitoneally injected them with 1 mg/kg CLO daily for 24 weeks, harvested their thoracic aortae, calculated the percentage of Sudan-IV-positive area to the whole vessel surface area, and found that CLO significantly decreased atherosclerosis in the thoracic aortae [10]. This evidence concerns the gene APOE and atherosclerosis.