AGO1 and benign prostatic hyperplasia: Mutating any of the binding sites abolished the suppression effect of agomir-34 on the reporter activity of the NlInR1 target sites (Fig 1D), indicating that both two binding sites are essential for the interaction between Nlu-miR-34 and NlInR1. Next, we performed RNA-binding protein immunoprecipitation (RIP) assay with the antibody-Ago1 (Argonaute 1) in wing buds of BPH nymphs.