VEGFA and hepatocellular carcinoma: We used ELISA kit to detect the effect of ASP on VEGF secretion in HCC cells (SK-Hep1 and Hep-3B) and found that the different concentrations of ASP (2.5, 5, and 10 mg/ml) significantly suppressed VEGF secretion by HCC cells in a concentration-dependent manner (Figure 5(a)).