In order to reveal the regulatory mechanisms of miR-142-3p relating with NF-κB signaling, the expression of IRAK1, Toll-like receptor 4 (TLR4), NF-κB p65 and phosphorylated NF-κB p65 (p-NF-κB p65) were detected in RA-HFLSs. Here, NFKB1 is linked to rheumatoid arthritis.