The univariate analysis based on the information of the 499 HNSCC patients obtained from TCGA indicated that MYOSLID expression (HR = 1.370, 95% CI [1.012–1.853], p = 0.041), gender (HR = 1.381, 95% CI [1.024–1.862], p = 0.034), and TNM stage (HR = 1.454, 95% CI [1.118–1.891], p = 0.005) were all significantly related to survival. This evidence concerns the gene MYOSLID and head and neck squamous cell carcinoma.