NOS2 and colitis: Charpentier et al. showed that supplementation of omega-3 PUFA significantly decreased colon inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, as well as IL-6 and leukotriene B4 production in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis [133].