Here, we demonstrated that SIRT3 deacetylase activity, as reflected by SOD2 acetylation but not its protein level, is associated with sensitivity of AML to chemotherapeutic agents including Ara‐C and daunorubicin, suggesting the existence of a subset of an active form of SIRT3, which may be due to its certain post‐translational modifications. Here, SIRT3 is linked to acute myeloid leukemia.