In our opinion, the attenuation of FUS acoustic intensities from the focal area (higher) to the “far field” (lower) zones may determine different osteosarcoma cell responses, which range from cell proliferation decrease (from 49 to 38.9 W/cm2) to improvement the maintenance of a subpopulation of living heterogeneous and immature cells (from 31.1 to 15.5 W/cm2) as demonstrated by the expression of early osteoblast markers RUNX2 and ALPL, which can clearly preserve bone tumor cells. This evidence concerns the gene ALPL and bone neoplasm.