Our optimized method achieved 100% concordance for the HER2 FISH NordiQC - Quality Control 2015 evaluation, 100% concordance for genotyping and clerical accuracy for the sarcoma panel containing CHOP (DDIT3), FUS, EWSR1 and MDM2 in the UK NEQAS International Quality Expertise 2016 evaluation. The gene discussed is MDM2; the disease is sarcoma.