As oncogenic KRAS is known as a major event in the IPMN-PDAC sequence, we further evaluated Pten and Rnf43 depletion in the context of constitutively overexpressed KRASG12D (KC) (Figure 3(g)) using the above described lentiviral knockdown system on KC ductal organoids. This evidence concerns the gene RNF43 and pancreatic intraductal papillary-mucinous neoplasm.