To investigate the causal relationship between autophagic flux inhibition by ECDD-S27 and cancer cell inhibition, we first measured whether autophagic flux inhibition by ECDD-S27 is correlated with cancer cell inhibition in GFP-LC3B expressing HT-29, HepG2, and HeLa cells treated with different concentrations of ECDD-S27 by HC image analysis. Here, MAP1LC3B is linked to cancer.