EGF and cancer: In UM-SCC47 and MDA-MB-231 cells, IQGAP1WT enhanced EGF-stimulated wound closure compared to control, mock-transfected cells, whereas IQGAP1∆IQ3 had no impact (Fig. 7a and Supplementary Fig. 8a), indicating that the IQ3 motif is required for the IQGAP1-mediated cancer cell migration.