To answer this, we measured the 50% inhibitory concentration (IC50) (concentration of miRNA needed to achieve half-maximal silencing of the tumor suppressor PDCD4) of miR-499 by cotransfecting it at increasing concentrations, together with psi-WT or the miR-21 mutant plasmid (psi-M2). The gene discussed is PDCD4; the disease is neoplasm.