The ELISA results showed that the plasma CORT levels in EtOHW rats increased significantly compared to those in the saline-treated control rats [F(3, 28) = 22.92, p < 0.001; saline-treated control group (56.04 ± 5.63, n = 8) vs. EtOH-treated control group (129.05 ± 10.20, n = 8), p < 0.001)], demonstrating the exacerbated anxiety behavior during EtOHW. The gene discussed is CORT; the disease is Anxiety.