However, the overall intensity is decreased in D cells transfected with ATG5-siRNA compared to those transfected with NT-siRNA (NT-siRNA-D-CQ-free versus ATG5-siRNA-D-CQ-free cells and NT-siRNA-D-CQ-blocked versus ATG5-siRNA-D-CQ-blocked cells) indicating a reduced AF (Figure 3A). Here, ATG5 is linked to atrial fibrillation.