MC3R and colorectal carcinoma: While a significant increase in intracellular ROS levels was detected in the three CRC cell lines as well as p53 R273H over-expressing HCT116 upon MC3 treatment (Figure 2A and Supplementary Figure 2A), HCT116 WT cells were found to exhibit higher ROS induction compared to the other two cell lines after long-term exposure to the highest concentration of MC3 (5 μM) (Figures 2A–C).