A total of 54 patients with HSP were enrolled in the study, including 36 (5 children) SPG4 (17 families), 5 SPG11 (4 families), 4 SPG5 (1 family), 4 CTX (4 families), 3 SPG7 (3 families), and 2 SPG3A (1 family) patients and 10 healthy control subjects. This evidence concerns the gene SPAST and hereditary spastic paraplegia.