Moreover, we found that the expression of PTEN was significantly decreased in the bladder cancer cells co-transfected with circSLC8A1 plasmids and miR-130b/miR-494 mimics, compared with the cells transfected with circSLC8A1 alone (Fig. 5d and e), which agreed with the results of cell function. This evidence concerns the gene PTEN and urinary bladder carcinoma.