Except for the age of 12 weeks, in the other three age groups, hepatic TNF‐α level in OVE26 male mice increased, whereas increased TNF‐α levels were found only in female OVE 26 mice at 4 weeks of age (Figure 6A), the results suggested that age and gender exert an impact on the inflammatory response in severe T1D. Here, TNF is linked to type 1 diabetes mellitus.