To explore the manner of cell death in brain tissue after stroke, we determined the number of propidium iodide-positive (PI+) and TdT-mediated dUTP nick end labelling (TUNEL+) cells in ischemic cortex at 6 h, 12 h, 1 day, 3 day, and 7 day after transient middle cerebral artery occlusion (tMCAO) (Fig. 1a). The gene discussed is DNTT; the disease is stroke disorder.