EXOC3 and osteosarcoma: We depleted the Exoc3 (Sec6 in worms) subunit of the complex in two mammalian cell lines, namely HeLa (a cervical cancer cell line) and U2OS (a human osteosarcoma cell line) cells via treatment with sequence-specific siRNAs (siExoc3) (Neto et al., 2013a) and immunostained to visualize microtubules (α-tubulin) and chromatin (DAPI) (Fig. 1A).