Furthermore, S100A1 is a potent diagnostic marker in clinical chordoma cases: zebrafish s100a1 was also the most significantly upregulated s100 gene in our hyperplastic zebrafish notochords, albeit under a slightly less stringent FDR threshold (logFC=2.32, P=0.006, FDR=0.096) (Fig. 5B,C, Table S1). Here, S100A1 is linked to chordoma.