Biochemical analyses were also performed to determine the subcellular distribution of AIF1; the results indicate that in MCF-7 breast cancer cells, AIF1 levels were increased in the cytosolic fractions of glucose/glutamine-deprived CHTM1-deficient cells (Fig. 3b, compare lanes 7&8) with concomitant decrease in the mitochondrial fractions (Fig. 3b, compare lanes 11&12). This evidence concerns the gene AIF1 and breast carcinoma.