Next, we showed that T cell receptor signaling pathway and RIG-1like receptor signaling pathway associated genes granzyme A (GZMA), perforin (PRF1), T cell receptor CD8A and DDX58 (RIG-1) were increased in MSI subtype colon cancer patients as presented through gene expression heatmaps in GSE13924, GSE18088 and GSE39084 datasets (Fig. 2c). This evidence concerns the gene PRF1 and malignant colon neoplasm.