Overall, in our data analysis, we identified multiple expression characteristics in the MSI subtype of colon cancer, for instance, inactivation of MMR system, TGFβ signaling pathway and Wnt-β-catenin signaling pathway, and the activation of PD-L1, IFN-γ signature, IRF1 transcription factor and T cell signaling pathway. Here, IRF1 is linked to malignant colon neoplasm.