Next, we showed that T cell receptor signaling pathway and RIG-1like receptor signaling pathway associated genes granzyme A (GZMA), perforin (PRF1), T cell receptor CD8A and DDX58 (RIG-1) were increased in MSI subtype colon cancer patients as presented through gene expression heatmaps in GSE13924, GSE18088 and GSE39084 datasets (Fig. 2c). The gene discussed is PRF1; the disease is colonic neoplasm.