TNF and infection: We next examined the cytokine content of total adipose tissue homogenate using a flow cytometry-based LegendPlex platform and found significant increases in IFNγ (p = 0.004), TNFα (p = 0.001), IL-1α (p = 0.0007), IL-6 (p<0.0001), and CCL2 (p<0.0001) (Fig 1C–1G) with infection in the adipose tissue on days 3 and 5 p.i. (days 3 and 5 are pooled in the figure).