The top pathways were as below: PPAR signaling pathway (Ppard, Fabp4, Scd1, Slc27a1, Cpt1a, Acsl1, Acadm), Adipocytokine signaling pathway (Prkaa1, Adipor1, Tnf, Nfkb1, Slc2a4, Cpt1a, Acsl1), AMPK signaling pathway (Prkaa1, Hmgcr, Adipor1, Acaca, Scd1, Cpt1a, Slc2a4), Non-alcoholic fatty liver disease (NAFLD) (Prkaa1, Adipor1, Tnf, nfkb1, Cebpa, Map3k5, Fas), Fatty acid metabolism (Acaca, Scd1, Cpt1a, Acsl1, Acadm), MAPK signaling pathway (Tnf, Nfkb1, Map3k5, Fas, Ntrk2), TNF signaling pathway (Tnf, Nfkb1, Map3k5, Fas), Insulin signaling pathway (Prkaa1, Acaca, Slc2a4, Ptbn1). The gene discussed is FAS; the disease is metabolic dysfunction-associated steatotic liver disease.