IGFBP3 and breast adenocarcinoma: In order to address the role of TM219 in autophagy activation mediated by IGFBP3, we inactivated TM219 protein from the genome of Vero and human primary breast adenocarcinoma epithelial MCF7 cells using TM219 RNA guide-CRSPR/Cas9 to confirm the importance of TM219 protein in this process.