L‐glutamine plays an essential role in promoting and maintaining the function of many organs, and studies have shown that L‐glutamine enhanced HSP expression in lung injury models.10 HSP70 is the major stress‐inducible member of the HSP family which is expressed at low levels in nearly all intracellular compartments.25 Our study demonstrated that L‐glutamine treatment increased the mRNA levels of HSP32, HSP70, and HSP110 in mouse brain after stroke, while HSP90 decreased compared to the saline group (Figure S2A, Table S2). This evidence concerns the gene HSP90B2P and stroke disorder.