Here, we found that p‐STAT3 and BDNF levels increased in parallel to HSP70 upregulation, indicating that L‐glutamine‐induced HSP70 was involved in the proliferation of astrocytes by activating p‐STAT3 pathway and promoted the secretion of BDNF after ischemic stroke (Figure 6D). This evidence concerns the gene STAT3 and ischemic stroke.