Activation of CD4+ T cells and CD8+ CTLs (cytotoxic T cells) is important for tumor rejection.37 To investigate whether rhCNB provokes antitumor T cell activity in vivo, WT C57BL/6 mice were injected intraperitoneally with different doses of rhCNB (10, 20, 40 mg/kg) or NaCl (as control) for 2 weeks, and splenocytes were isolated and harvested to detect CTLs by FACs. The gene discussed is CD4; the disease is neoplasm.