To assess whether the antitumor effects of rhCNB is mediated by TLR4 in vivo, LLC cells suspensions (1 × 106 cells) were transplanted into the right armpits of C57BL/6 TLR4 knockout mice and their littermates, and they were given daily intraperitoneal injections (0.2 mL) of normal saline or rhCNB 20 mg/kg, again rhCNB caused a progressive reduction in tumor size in the wild‐type mice with increasing length of administration (Figure 7A). Here, TLR4 is linked to neoplasm.