TRPA1 and myocardial infarction: However, compared to WT MI mice, KO MI mice had significant reductions in fibrotic scarring, as determined by Masson's trichrome staining (P < 0.05, P < 0.01, and P < 0.05) (Figures 1(f) and 1(g)); collagen content, as reflected by picrosirius red staining (Figures 1(h), 1(i), and 1(j)); and TGF-β levels in heart tissue lysate supernatant (Figure 1(k)) (P < 0.05, P < 0.05, and P < 0.01, respectively), whereas CA pretreatment aggravated cardiac fibrosis (Figures 1(f) and 1(j)), indicating that TRPA1 is necessary for MI injury-induced cardiac fibrosis.