FBXO22 and cancer: To study the relationship between FBXO22 and LKB1, two pairs of shRNAs (shFBXO22#1 and shFBXO22#2) specifically against FBXO22 were generated to knockdown FBXO22 along with a control shRNA (shNC) in 293T, H322, and H1299, we did not observe significant changes in LKB1 protein level upon FBXO22 knockdown in these cells (Fig. 4a, b) and other cancer cells (Fig. S1A).