The results showed that the phosphorylation of AMPKα was induced in FBXO22 knockout MEF cells (Fig. 5a), and knockdown of FBXO22 increased while induced FBXO22 expression decreased AMPK phosphorylation in LKB1-proficient lung cancer cells (Fig. 5b, c), suggesting FBXO22 is critical for maintaining LKB1 activity. This evidence concerns the gene PRKAA1 and lung carcinoma.