NLRP3 and Caspase-1 colocalization events were significantly decreased in the P47 phox KO group when compared to CHOW + MC and NAFLD + MC groups suggesting a decreased NLRP3 inflammasome activation (p < 0.05)(Fig. 9Bv–viii,D). Here, CASP1 is linked to metabolic dysfunction-associated steatotic liver disease.