The levels of another tight junction protein Occludin increased significantly in the CHOW + MC (P47 phox KO) group when compared to CHOW + MC group, and also there was a significant increase in NAFLD + MC (P47 phox KO) group when compared to NAFLD + MC group suggesting that NOX2 was important for the alterations of these proteins crucial for maintaining gut barrier integrity (p < 0.05)(Fig. 11Bv–viii,D). This evidence concerns the gene OCLN and metabolic dysfunction-associated steatotic liver disease.