CYBB and metabolic dysfunction-associated steatotic liver disease: IL1β protein levels as measured by immunohistochemistry decreased significantly in the p47 phox KO group when compared to CHOW + MC and NAFLD + MC groups suggesting that NOX-2 was required for the inflammasome activation and IL1β release in the diseased intestine (Fig. 10Ai–iv,B) (P < 0.05).