After successfully porting the bulk library prep protocol to Fluidigm's C1 single cell instrument, we assessed the performance of the single cell total RNA-seq protocol through three distinct experiments in which nutlin-3, JQ1 or doxycycline was used to treat NGP, SK-N-BE-2C and SHSY5Y-MYCN-TR neuroblastoma cell lines, respectively (with vehicle treated cells as control) (Figure 1). The gene discussed is MYCN; the disease is neuroblastoma.