mRNA analysis of the EMS control group resulted in a significant decrease in main related factors implicated in insulin signaling pathway (Fig. 7a); indeed, expression of GLUT4, insulin receptor (IR), insulin receptor substrate (IRS), AKT, and SREBP1C were significantly downregulated under metabolic syndrome condition (p < 0.05; p < 0.001). The gene discussed is IARS1; the disease is metabolic syndrome.