To test the role of RhoA and RhoC in M2a-induced breast cancer cell migration, we stimulated MDA-231 or SUM-149 wild type (WT), RhoA CRISPR-Cas9 knockout (ΔRhoA), or RhoC CRISPR-Cas9 knockout (ΔRhoC) with either base media or M2a conditioned media and monitored wound closure over 24 h. The gene discussed is RHOC; the disease is breast carcinoma.