In the laryngeal cancer and paracancerous mucosa specimens, gene chip data analysis showed that the FOXN3 gene was significantly down-regulated while the ADAM12 gene expression was significantly up-regulated, and subsequent real-time quantitative PCR for the validation of the mRNA expressions in 24 cases of laryngeal cancer and 17 cases of normal mucosa showed that the results were consistent with the those of detection with gene chip. This evidence concerns the gene FOXN3 and laryngeal carcinoma.