Evaluation of PDL1 expression on macrophages and on CD163 negative cells at the tumor site via image cytometry suggest that while Tadalafil and/or polyICLC vaccine are effective in reducing PDL1+macrophage at the tumor edge, the increase of CD69+T cells within the tumor promotes (Figure 5) the expression of this inhibitory molecules on CD163−cells (Figure 6). The gene discussed is CD163; the disease is neoplasm.