Transcriptional analysis by quantitative real-time PCR with reverse transcription (qRT-PCR) of tumor tissue from mice at day 15–17 after tumor cell inoculation revealed a significant increase of tumor necrosis factor alpha (Tnfα), interferon-γ (Ifnγ) and a tendency of increased nitric oxide synthase 2 (Nos2) expression (p = 0.0549), whereas transcripts for interleukin (IL) 10 (Il10) and granulocyte macrophage-colony stimulating factor 2 (Csf2) remained unchanged (Figure 2A). This evidence concerns the gene TNF and neoplasm.