AGT and Alzheimer disease: In vitro experiments also demonstrated that treatments of rat primary neurons, astrocytes, and human neuroblastoma cells with 1–10 μM 27-OHC stimulate the production of angiotensinogen, the precursor of Ang I. Moreover, in AD the activity of ACE correlates with 27-OHC levels both in plasma and CSF (Mateos et al., 2011a), although ACE levels have been shown to be reduced in the CSF (Miners et al., 2009).