By western blot analysis, we found an up-regulation of HK2 protein expression in ovarian cancer cell lines (OVCAR-3, OVCA429, OVCA433, OC316, ES-2, TOV21G, A2780S, and A2780CP), compared to normal ovarian epithelial cell lines (HOSE 6-3 and HOSE 11-12) (Figure 1D). This evidence concerns the gene HK2 and ovarian cancer.