To ensure that the expression of perforin and GzmB detected in in vitro MDSCs is not an artifact of the in vitro culture and is representative for different tumor models, we studied MDSCs isolated from the tumor and spleen of mice bearing B16F10 melanoma, CT26 colorectal carcinoma, E.G7-OVA T-cell lymphoma, and 4T1 mammary carcinoma. The gene discussed is PRF1; the disease is melanoma.