As shown in Figure 5A, both vGluT1 and GABA -positive neurons could be observed in the ASCL1-mediated glioma cell-to-neuron system, while no ChAT signal was detected in the culture, suggesting that ASCL1 reprogrammed glioma cells into inhibitory/excitatory interneurons but not motor neurons. The gene discussed is ASCL1; the disease is glioma.