INS and liver cancer: We first assessed total tyrosine phosphorylation level of IRTKS during insulin stimulation, showing that the tyrosine phosphorylation level of IRTKS was increased in 5 min and attenuated in 90 min after insulin stimulation in human HEK293T cells (Figure 4A) as well as liver cancer cell lines SK-Hep-1, Huh7, and HepG2 (Figure 4B–G).