Experimental studies showed an increased concentration of AEA, NAPE-PLD, and CB1 and low expression of FAAH in the adipose tissue, while the reverse was observed in prebiotic treated animals revealing the fact that eCB activation leads to obesity and intervention of eCB upregulation is beneficial [114, 116]. This evidence concerns the gene CNR1 and obesity due to melanocortin 4 receptor deficiency.