To investigate the effects of AKT and PRAS40 activity in PC cell lines, PC-3, LnCAP, and VCAP cells were treated with BEZ235, a highly specific AKT inhibitor [43–45], and GDC-0068, a PRAS40 inhibitor [46, 47]. The gene discussed is AKT1S1; the disease is pachyonychia congenita.