As a complementary approach, GFP-RhoA WT and a generated chimera of Ran fused with KillerRed-membrane (Ran-KillerRed) were transiently expressed in Ran KD TOV-112D cells to exclusively target Ran to the PM, confirming the role of Ran in the recruitment of RhoA to the PM/ruffle and the effect on cancer cell proliferation and invasion. The gene discussed is TNFRSF10B; the disease is cancer.